[BioC] package ‘hugene10stv1.r3cdf’ or ‘hugene10stv1’ is not available
Hooiveld, Guido
Guido.Hooiveld at wur.nl
Mon Jun 18 11:46:28 CEST 2012
Hi,
First of all, please be aware that for the Gene ST arrays 'unofficial' CDFs are provided. That is, although Affymetrix released a CDF file for these arrays, these should be considered experimental.
The preferred way of analysing these arrays is through the library 'oligo' or 'XPS'. They make use of all files provided by Affymetrix (pgf, clf, annotation CSV, etc). Please see the respective vignettes for more details.
Having said this, for the analysis you are currently performing you need:
http://www.bioconductor.org/packages/2.10/data/annotation/html/hugene10stv1cdf.html
HTH,
Guido
---------------------------------------------------------
Guido Hooiveld, PhD
Nutrition, Metabolism & Genomics Group
Division of Human Nutrition
Wageningen University
Biotechnion, Bomenweg 2
NL-6703 HD Wageningen
the Netherlands
tel: (+)31 317 485788
fax: (+)31 317 483342
email: guido.hooiveld at wur.nl
internet: http://nutrigene.4t.com
http://scholar.google.com/citations?user=qFHaMnoAAAAJ
http://www.researcherid.com/rid/F-4912-2010
-----Original Message-----
From: bioconductor-bounces at r-project.org [mailto:bioconductor-bounces at r-project.org] On Behalf Of suparna mitra
Sent: Monday, June 18, 2012 11:14
To: bioconductor at r-project.org
Subject: [BioC] package ‘hugene10stv1.r3cdf’ or ‘hugene10stv1’ is not available
Hi members,
I'm trying for quite some time to get an analysis started for affy microarray files which has HuGene-1_0-st-v1.
justrma step worked.
> eset_justrma
ExpressionSet (storageMode: lockedEnvironment)
assayData: 32321 features, 18 samples
element names: exprs, se.exprs
protocolData
sampleNames: MC1_(HuGene-1_0-st-v1).CEL MC10_(HuGene-1_0-st-v1).CEL ...
MC9_(HuGene-1_0-st-v1).CEL (18 total)
varLabels: ScanDate
varMetadata: labelDescription
phenoData
sampleNames: MC1_(HuGene-1_0-st-v1).CEL MC10_(HuGene-1_0-st-v1).CEL ...
MC9_(HuGene-1_0-st-v1).CEL (18 total)
varLabels: sample
varMetadata: labelDescription
featureData: none
experimentData: use 'experimentData(object)'
Annotation: hugene10stv1
But I am not able to do the annotation. I tried many possible options.
I keep getting this Error.
> biocLite("hugene10stv1.r3cdf")
Using R version 2.12.2, biocinstall version 2.7.7.
Installing Bioconductor version 2.7 packages:
[1] "hugene10stv1.r3cdf"
Please wait...
Warning message:
In getDependencies(pkgs, dependencies, available, lib) :
package ‘hugene10stv1.r3cdf’ is not available
> library("annotate")
> library("hugene10stv1")
Error in library("hugene10stv1") :
there is no package called 'hugene10stv1'
> library("hugene10stv1.db")
Error in library("hugene10stv1.db") :
there is no package called 'hugene10stv1.db'
> biocLite("hugene10stv1.r3cdf", type = "source")
Using R version 2.12.2, biocinstall version 2.7.7.
Installing Bioconductor version 2.7 packages:
[1] "hugene10stv1.r3cdf"
Please wait...
Warning message:
In getDependencies(pkgs, dependencies, available, lib) :
package ‘hugene10stv1.r3cdf’ is not available
Can anybody please help. Thanks a lot in advance.
Best wishes,
Suparna.
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