[BioC] affy : ReadAffy() fails on HuGene-1_1-st-v1 chips

Steven Osselaer [guest] guest at bioconductor.org
Tue Jan 24 10:42:41 CET 2012


Reading HuGene-1_1-st-v1 CEL files results in an error message about  incorrect dimensions of the first CEL file of the list

TRANSCRIPT :

R version 2.14.1 (2011-12-22)
Copyright (C) 2011 The R Foundation for Statistical Computing
ISBN 3-900051-07-0
Platform: x86_64-unknown-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

  Natural language support but running in an English locale

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(affy)
Loading required package: Biobase

Welcome to Bioconductor

  Vignettes contain introductory material. To view, type
  'browseVignettes()'. To cite Bioconductor, see
  'citation("Biobase")' and for packages 'citation("pkgname")'.

> sessionInfo()
R version 2.14.1 (2011-12-22)
Platform: x86_64-unknown-linux-gnu (64-bit)

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=C                 LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
[1] affy_1.32.0    Biobase_2.14.0

loaded via a namespace (and not attached):
[1] affyio_1.22.0         BiocInstaller_1.2.1   preprocessCore_1.16.0
[4] zlibbioc_1.0.0
> celFiles <- list.files(pattern="CEL$")
> celFiles
 [1] "27002.CEL" "27003.CEL" "27004.CEL" "27005.CEL" "27006.CEL" "27007.CEL"
 [7] "27008.CEL" "27009.CEL" "27010.CEL" "27011.CEL" "27012.CEL" "27013.CEL"
[13] "27014.CEL" "27015.CEL" "27016.CEL" "27017.CEL" "27018.CEL" "27019.CEL"
[19] "27020.CEL" "27021.CEL" "27022.CEL" "27023.CEL" "27024.CEL" "27025.CEL"
[25] "27026.CEL" "27027.CEL" "27028.CEL" "27029.CEL" "27030.CEL" "27031.CEL"
[31] "27032.CEL" "27033.CEL" "27034.CEL" "27035.CEL" "27036.CEL" "27037.CEL"
[37] "27038.CEL" "27039.CEL" "27040.CEL" "27041.CEL" "27042.CEL" "27043.CEL"
[43] "27044.CEL" "27045.CEL" "27046.CEL" "27047.CEL" "27048.CEL" "27049.CEL"
[49] "27050.CEL" "27051.CEL" "27052.CEL" "27053.CEL" "27054.CEL" "27055.CEL"
[55] "27056.CEL" "27057.CEL" "27058.CEL" "27059.CEL" "27060.CEL" "27061.CEL"
[61] "27062.CEL" "27063.CEL" "27064.CEL" "27065.CEL" "27066.CEL" "27067.CEL"
[67] "27068.CEL" "27069.CEL" "27070.CEL" "27071.CEL" "27072.CEL" "27073.CEL"
[73] "27074.CEL" "27075.CEL" "27076.CEL" "27077.CEL" "27078.CEL" "27079.CEL"
[79] "27080.CEL" "27081.CEL" "27082.CEL" "27083.CEL" "27084.CEL" "27085.CEL"
[85] "27086.CEL" "27087.CEL" "27088.CEL" "27089.CEL" "27090.CEL" "27091.CEL"
[91] "27092.CEL" "27093.CEL" "27094.CEL" "27095.CEL" "27096.CEL"
> rawData <- ReadAffy(filenames=celFiles)
Error in read.affybatch(filenames = l$filenames, phenoData = l$phenoData,  :
  Cel file 27002.CEL does not seem to have the correct dimensions
> traceback()
3: .Call("read_abatch", filenames, rm.mask, rm.outliers, rm.extra,
       ref.cdfName, dim.intensity[c("Rows", "Cols")], verbose, PACKAGE = "affyio")
2: read.affybatch(filenames = l$filenames, phenoData = l$phenoData,
       description = l$description, notes = notes, compress = compress,
       rm.mask = rm.mask, rm.outliers = rm.outliers, rm.extra = rm.extra,
       verbose = verbose, sd = sd, cdfname = cdfname)
1: ReadAffy(filenames = celFiles)


 -- output of sessionInfo(): 

R version 2.14.1 (2011-12-22)
Platform: x86_64-unknown-linux-gnu (64-bit)

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=C                 LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
[1] affy_1.32.0    Biobase_2.14.0

loaded via a namespace (and not attached):
[1] affyio_1.22.0         BiocInstaller_1.2.1   preprocessCore_1.16.0
[4] zlibbioc_1.0.0


--
Sent via the guest posting facility at bioconductor.org.



More information about the Bioconductor mailing list