[BioC] How remove controls probes - Affymetrix Gene
Sophie Lamarre [guest]
guest at bioconductor.org
Thu Jan 12 17:01:54 CET 2012
Hello,
I work on affymetrix mouse gene 1.0 ST.
I want to know how remove controls probes on Affymetrix Gene.
In my studie, I downloaded two files on Affymetrix website:
- The Probeset Annotations, CSV Format, Release 32
file (NetAffx Analysis Center -> Exon/Gene -> NetAffy Query -> "Mouse Gene 1.0 ST")
- The Transcript Cluster Annotations, CSV, Release 32 file (NetAffx Analysis Center -> Exon/Gene -> NetAffy Query -> "Mouse Gene 1.0 ST")
With the first, I had 28 856 main probesets and with second 28 350 main probesets.
I add that I run a RMA option "core" with the oligo package. I just want a probe for a probeset.
Which file I have to use? (I did'nt use the command getNetAffy because I realized that I lost more probesets! (There were only 28 270 main probes)).
Thank you for advance for your answer and your help.
Sophie LAMARRE
-- output of sessionInfo():
R version 2.13.0 (2011-04-13)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=French_France.1252 LC_CTYPE=French_France.1252 LC_MONETARY=French_France.1252
[4] LC_NUMERIC=C LC_TIME=French_France.1252
attached base packages:
[1] grid stats graphics grDevices utils datasets methods base
other attached packages:
[1] pd.mogene.1.0.st.v1_3.2.0 oligo_1.16.2 preprocessCore_1.14.0
[4] GOstats_2.18.0 RSQLite_0.9-4 DBI_0.2-5
[7] graph_1.30.0 Category_2.18.0 genefilter_1.34.0
[10] gplots_2.8.0 caTools_1.12 bitops_1.0-4.1
[13] gdata_2.8.2 gtools_2.6.2 geneplotter_1.30.0
[16] lattice_0.19-23 annotate_1.30.0 AnnotationDbi_1.14.1
[19] RColorBrewer_1.0-5 ArrayTools_1.12.0 affy_1.30.0
[22] limma_3.8.3 oligoClasses_1.14.0 Biobase_2.12.2
loaded via a namespace (and not attached):
[1] affxparser_1.24.0 affyio_1.20.0 Biostrings_2.20.3 bit_1.1-7 ff_2.2-3
[6] GO.db_2.5.0 GSEABase_1.14.0 IRanges_1.10.6 RBGL_1.28.0 splines_2.13.0
[11] survival_2.36-5 tools_2.13.0 XML_3.4-2.2 xtable_1.5-6
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