[BioC] about the quality score

[wang peter]

i have data, like this
@HWI-ST397_0000:5:1101:1110:2156#NTNTCG/1
CAAGGAGCAGAGTGAGGTCTCTCCGCAGAGCGCCAAGGCAAAGCAGATGGTGGAGCTGCTCTACGAGACGGCGCACATTGCGTCTGGATTCCCGCCTG
+HWI-ST397_0000:5:1101:1110:2156#NTNTCG/1
gdgggegc_gffdeeggdgfggggdgbfadaeeeeefaefgcebebdffebbbbcg`gdgfgeeebgbddcbccbfdffcdcadbW_]]`]dd_bb\^
reads <- readFastq(fastqfile);
seqs <- sread(reads)
it seems phred+64 system

can i use the function:
qual <- PhredQuality(quality(quality(reads)))
i think can only read phred+32 system

-- 
shan gao
Room 231(Dr.Fei lab)
Boyce Thompson Institute
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