[BioC] Problem in analysing Agilent single color data using LIMMA
James W. MacDonald
jmacdon at uw.edu
Fri Apr 27 14:50:05 CEST 2012
On 4/27/2012 1:48 AM, Muralidharan V wrote:
> Hai,
>
> I had few questions regarding the analysis of Agilent microarray data
> using the LIMMA pacakge.
>
> Is it possible to analyse the single color data using the R+Bioconductor
> using the LIMMA package? We dont have the replicates instead of that we
> have pooled the samples before sending for the analysis.
> So is it possible to use the LIMMA package without having any replicates.
You can analyze single color Agilent microarray data with limma.
However, if you don't have replicates you cannot do anything more than
compute fold change (which being trivial, is not something that limma does).
Best,
Jim
>
>
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--
James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
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