[BioC] Can beadarray be use to analyze iScan raw data without perBeadFile.txt files?

Anna [guest] guest at bioconductor.org
Wed Apr 11 02:19:01 CEST 2012


Hi,

I am interested in using the beadarray package to process the raw data generated by the Illumnia's iScan.  Unfortunately,  the settings for the Illumina's iScan was not set correctly during the run to output the perBeadFile.txt files.  The following files were generated from the run:

5456816021_qc.txt
5456816021_R01C01_1_Focus_scan#1_swath#1_point#1_try#1.jpg
5456816021_R01C01_1-Swath1_Grn.jpg
5456816021_R01C01_1-Swath1_Red.jpg
5456816021_R01C01_1-Swath2_Grn.jpg
5456816021_R01C01_1-Swath2_Red.jpg
5456816021_R01C01_1.xml
5456816021_R01C01_2_Focus_scan#1_swath#1_point#1_try#1.jpg
5456816021_R01C01_2-Swath1_Grn.jpg
5456816021_R01C01_2-Swath1_Red.jpg
5456816021_R01C01_2-Swath2_Grn.jpg
5456816021_R01C01_2-Swath2_Red.jpg
5456816021_R01C01_2.xml

Is it still possible to use beadarray to analyze the raw data outputs from the iScan?  Thanks in advance for any help.

Anna

 -- output of sessionInfo(): 

R version 2.15.0 (2012-03-30)
Platform: i386-apple-darwin9.8.0/i386 (32-bit)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] BiocInstaller_1.4.3

loaded via a namespace (and not attached):
[1] tools_2.15.0


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