[BioC] goseq problem
Naomi Altman
naomi at stat.psu.edu
Mon Mar 28 16:10:49 CEST 2011
After using edgeR to find differentially expressed genes, I tried to
follow the instructions in goseq to correct for length bias.
top$table[1:10,1]
[1] "ENSG00000138131" "ENSG00000204616" "ENSG00000204876"
"ENSG00000118972" "ENSG00000143546" "ENSG00000163993"
"ENSG00000012223" "ENSG00000188257"
[9] "ENSG00000140403" "ENSG00000059804"
geneLengths=getlength(top$table[,1],'hg19','ensGene')
geneLengths[1:10]
[1] 3672.0 2033.5 2675.5 3021.0 454.0 512.0 1813.0 911.5 2877.0 3504.0
(so far, so good)
pwf=nullp(top$table[,1],'hg19','ensGene')
Loading hg19 length data...
Error in if (matched_frac == 0) { : missing value where TRUE/FALSE needed
> sessionInfo()
R version 2.12.2 (2011-02-25)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United
States.1252 LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C LC_TIME=English_United
States.1252
attached base packages:
[1] tools grid stats graphics grDevices
utils datasets methods base
other attached packages:
[1] goseq_1.2.0 geneLenDataBase_0.99.5
BiasedUrn_1.03 qvalue_1.24.0 sagenhaft_1.20.0
SparseM_0.86
[7]
edgeR_2.0.5 hexbin_1.24.1 DESeq_1.2.1
locfit_1.5-6 lattice_0.19-17 akima_0.5-4
[13] Biobase_2.10.0
loaded via a namespace (and not attached):
[1] annotate_1.28.1 AnnotationDbi_1.12.0
Biostrings_2.18.4 BSgenome_1.18.3 DBI_0.2-5
genefilter_1.32.0
[7]
geneplotter_1.28.0 GenomicRanges_1.2.3 IRanges_1.8.9
limma_3.6.9 Matrix_0.999375-46 mgcv_1.7-3
[13]
nlme_3.1-98 RColorBrewer_1.0-2 RCurl_1.5-0.1
RSQLite_0.9-4 rtracklayer_1.10.6 splines_2.12.2
[19]
survival_2.36-5 tcltk_2.12.2 XML_3.2-0.2
xtable_1.5-6
>
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