[BioC] limma design experiment

[Gordon K Smyth]

Dear Luis,

Are you asking how to construct a design matrix?  Why not

   design <- modelMatrix(targets, ref="C")

as described in the limma User's Guide?

Best wishes
Gordon

> Date: Tue, 15 Mar 2011 14:52:05 -0400
> From: Luis Z <luisgls at gmail.com>
> To: bioconductor at r-project.org
> Subject: [BioC] limma design experiment
> Message-ID:
>
> Hi everyone,
>
> I would like to receive a suggestion on the design model for this
> experiment.
>
> There are 5 different RNA pool families. Each one with three replicates
> (Technical?), coming from individuals that receive three different
> treatments, a control treatment and T1, T2. The experiment were made :
>                  fam    Cy3   Cy5
> slide 1         F1    T1   C
>                  F1    T1   C
>                  ....
>                  F5     T1   C
>                  F1     T2   C
>                  ...
>                  F5      T2   C
>
> What would you suggest?
>
> Thanks,
>
> Luis
> -- 
> Luis Zapata Ortiz
> Molecular Biotechnology Engineering
> Laboratory of Bioinformatics and Mathematics of the Genome, LBMG.
> Center of Mathematical Modeling of the University of Chile.
> Av. Blanco Encalada 2120
> 56-2-9784551

______________________________________________________________________
The information in this email is confidential and intend...{{dropped:4}}