[BioC] GLMs in DESeq

[Seanna McTaggart]

I am using DESeq to determine DE of an RNA-Seq project that has multiple 
genotypes
exposed to different treatments. I would like to use the GLM 
functionality to partition
the variance in the count data between genotype and treatment. However, 
when I follow
the suggestions of Simon (Bioconductor Digest, Vol 96, Issue 9), neither 
model
(fit0 or fit1) is reaching convergence, and I was wondering if it was 
possible for me to
increase the number of iterations to see if this would help out. 
Otherwise I would appreciate
any advice on how to proceed.

Many thanks for your time,

Seanna

-- 

Seanna McTaggart
Centre for Immunity, Infection and Evolution
School of Biological Sciences
Ashworth Laboratories
University of Edinburgh
Edinburgh EH9 3JT
Scotland, UK

Tel +44 131 650 8682
Fax +44 131 650 6564
E-mail: smctagga at staffmail.ed.ac.uk

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