[BioC] fold change after gcrma()
yotsawat pomyen
yotsa at yahoo.com
Mon Jun 13 15:46:02 CEST 2011
Dear All,
I normalised and summarised my data using gcrma() routine and have the
expression values for each probeset. I now want to do the comparison between two
classes of the samples (i.e. fold change between the classes). One thing I'm not
sure is how to compare them because, as I read from the gcrma paper, the
expression values after the gcrma() routine are log(intensity) after background
adjustment.
My question is: Do I have to convert the expression values from gcrma() back to
raw values by e^(values) before calculate the fold change between the two
classes that I have?
Best
Yot
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