[BioC] backgroundCorrect offset value
siddavatam at gmail.com
Mon Aug 29 04:17:01 CEST 2011
Dear Dr. Smyth,
Thank you very much for your detailed response. I am going to read the
references. I understand why I am getting more differential genes with bigger
offset value. Basically we are bringing the variance close to zero (with more
uniformity across probes).
Are you suggesting to delete the probes before doing the background correct?
If yes, is there any limit on the maximum number of probes to be deleted?
In the variance stabilization, which maximum value (approximately) of
fit$df.prior is considered high enough to call a good variance stabilization is
Appreciate your time and help.
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