[BioC] RMA: Affy MoEx and background correction
Peter Saffrey
pzs at dcs.gla.ac.uk
Wed Nov 24 14:27:04 CET 2010
I am using the affy library to compute RMA values on mouse exon arrays,
using the mouseexonpmcdf from here:
http://xmap.picr.man.ac.uk/download#mmcdf
It works fine, but I wanted to check some of the details of the
implementation and hoped somebody on this list could help.
As I understand it, the background correction step of the RMA
calculation usually works on the mismatch (MM) probes on the array.
However, on the exon array, there are no MM probes, so the calculation
is done based on a separate pool of background probes. There is a
description here:
http://media.affymetrix.com/support/technical/whitepapers/exon_background_correction_whitepaper.pdf
Is this seamlessly happening behind the scenes when I run RMA with the
mouse exon CDF or do I need to do something else to ensure the proper
background correction is taking place?
Peter
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