[BioC] ShortRead information
Jennifer Dupiot
jennifer.dupiot at bordeaux.inra.fr
Thu May 27 15:00:12 CEST 2010
Dear all,
I am new to bioconductor and little experienced in R.
I work on illumina data, and would like to analyse Run with ShortRead.
I have questions about using ShortRead :
* Is it necessary to use whole illumina run folder to do quality
analysis ? or only fastq files could be sufficients ?
* I tried to run following command :
>
sp<-SolexaPath("/media/data/CSE/Delivery_2010.02.26/17.2_FlowCell61BWR_75ntX75nt_3.7GB/",analysisPath="/media/data/CSE/Delivery_2010.02.26/17.2_FlowCell61BWR_75ntX75nt_3.7GB/17.2_6_1_sequence.fastq/")
> ap<-analysisPath (sp)
> reads<-readFastq(ap,"fastq")
and it returns :
"Erreur dans .local(dirPath, pattern, ...) :
cannot open file
/media/data/CSE/Delivery_2010.02.26/17.2_FlowCell61BWR_75ntX75nt_3.7GB/17.2_6_1_sequence.fastq//s_6_1_sequence.fastq"
Do I do something wrong ?
Do I miss something ?
Could you give me some clues on this ?
For information, computer configuration is :
ubuntu 9.10
R 2.9.2
ShortRead 1.2.1
Thanks for your help.
Regards,
Jennifer
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