[BioC] Human Gene ST using oligo package

Javier Pérez Florido jpflorido at gmail.com
Wed May 19 21:37:50 CEST 2010


Dear list,
Some time ago I used the oligo package to read and pre-process (rma) a 
set of Human Gene ST 1.0 CEL files and it was OK.

Now, I have resumed my work and with the new version of oligo and R 
(below is my session info), the same code doesn't work (the new oligo 
vignette is quite different to the one I used before). I have downloaded 
first the pd.hugene.1.0.st package from bioconductor and then I built it 
myself (to check if it was a problem of the annotation package) and the 
same error appears:

OligoRaw<-read.celfiles(filenames=list.celfiles())
OligoEset<-rma(OligoRaw,target="core") # It is a GeneFeatureSet object
Error in function (classes, fdef, mtable)  :
   unable to find an inherited method for function "probeNames", for 
signature "GeneFeatureSet"

Any suggestions?
Thanks,
Javier


R version 2.11.0 (2010-04-22)
i386-pc-mingw32

locale:
[1] LC_COLLATE=Spanish_Spain.1252  LC_CTYPE=Spanish_Spain.1252    
LC_MONETARY=Spanish_Spain.1252 LC_NUMERIC=C
[5] LC_TIME=Spanish_Spain.1252

attached base packages:
  [1] grid      tools     tcltk     stats     graphics  grDevices 
utils     datasets  methods   base

other attached packages:
  [1] hugene10sttranscriptcluster.db_5.0.1 
org.Hs.eg.db_2.4.1                   RColorBrewer_1.0-2
  [4] gplots_2.7.4                         
caTools_1.10                         bitops_1.0-4.1
  [7] gdata_2.8.0                          
gtools_2.6.2                         oneChannelGUI_1.14.2
[10] RaExExonProbesetLocation_1.0.0       
MoExExonProbesetLocation_1.0.0       HuExExonProbesetLocationHg19_0.0.2
[13] edgeR_1.6.2                          
IRanges_1.6.0                        preprocessCore_1.10.0
[16] GOstats_2.14.0                       
graph_1.26.0                         Category_2.14.0
[19] AnnotationDbi_1.10.0                 
tkWidgets_1.26.0                     DynDoc_1.26.0
[22] widgetTools_1.26.0                   
affylmGUI_1.22.0                     affyio_1.16.0
[25] affy_1.26.0                          
limma_3.4.0                          pd.hugene.1.0.st.v1_0.0.1
[28] pdInfoBuilder_1.12.0                 
oligo_1.12.0                         oligoClasses_1.10.0
[31] affxparser_1.20.0                    
RSQLite_0.8-4                        DBI_0.2-5
[34] Biobase_2.8.0

loaded via a namespace:
  [1] annotate_1.26.0   Biostrings_2.16.0 genefilter_1.30.0 
GO.db_2.4.1       GSEABase_1.10.0   RBGL_1.24.0       splines_2.11.0
  [8] survival_2.35-8   XML_3.1-0         xtable_1.5-6



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