[BioC] Limma: technical replicates in multi-group design

Kasper Daniel Hansen kasperdanielhansen at gmail.com
Mon Mar 29 18:09:57 CEST 2010

The fact that it is pooled does not help much.  I suggest reading
(PNAS 2005, "On the utility of pooling biological samples in
microarray experiments")


Specifically, I suggest looking at Figure 5, which is rather interesting.


On Fri, Mar 26, 2010 at 7:47 PM, Adam Kiezun <akiezun at gmail.com> wrote:
>> If I understand it correctly, there is no
>> biological replication at all in this experiment.  In that situation, there
>> is no way to assess statistical significance relative to biological
>> variation.
> Thanks Gordon, Mike, Wei, James,
> I'm not sure if it changes the situation but each of the 3
> samples/groups comes from RNA pooled from 5 mice.
> ./adam
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