[BioC] how to generate a data frame containing all the coefficients of the different contrasts, the p-value and the adjusted p-value ?

[J.delasHeras at ed.ac.uk]

Quoting Benoit Loup <benoit.loup at jouy.inra.fr>:

> Hi to all,
>
> I have a microarray experiment with 10 different contrasts and I would
> like to generate a dataframe containing all the informations exported
> with the function toptable for all the the genes (no restrictions on
> p-value and logFC but with BH adjustment).
>
> For 1 specific contrast I proceed like that :
> res=toptable(coef=1,number=15120,fit=fit2,genelist=Ebgnorm$genes[isGene,],adjust.method="BH",A=fit2$Amean,eb=fit2)
> res.summary=data.frame(res$ProbeUID,res$ProbeName,res$GeneName,res$Description,res$P.Value,res$adj.P.Val,res$logFC,exp(res$logFC*log(2)))
>
> and after I add to this dataframe the normalized log intensities and weights:
> lg=data.frame(Ebgnorm$genes$ProbeUID[isGene],Ebgnorm$genes$ProbeName[isGene],Ebgnorm$E[isGene,],Ebgnorm$weights[isGene,])
> res2=res.summary[order(res.summary$ProbeUID),]
> lg2=lg[order(lg$ProbeUID),]
> res2=cbind(res2,lg2[,3:18])
> write.table(res2,file="result_Weights_and_Intensities.txt",sep="\t",quote=FALSE,col.names=TRUE,row.names=FALSE)
>
> It works very well for one contrast but now, I would like to do that
> for all the contrasts at the same time.
> I tried the functions write.fit and topTableF but, with the first one I
> cannot have the adjusted p-values and for the second one, there is only
> one column of adjusted p-value (with method="separate" and "global")
> and not one for each contrast.
>
> So, is there any other function to solve my problem or will I have to
> perform the exportation of all contrasts one by one and then
> concatenate them ?
>
> Thank in advance for your help.
>
> Benoit

Hi Benoit,

I normally create a list with each individual contrast information  
(the result of 'topTable', not-ordered, so all probesets in teh same  
order) as a separate component. I like 'topTable' because it gives me  
all the info I want. Then I merely compile a large table by combining  
the columns containing first the annotation info, and then the  
different contrasts data (I normally just export the log2 ratios and  
adjusted p values at this stage).
By putting them in a list you can automate the big table generation no  
matter how many contrasts you have.
It may not be the most elegant form, but that's what I do.

Jose


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Dr. Jose I. de las Heras                      Email: J.delasHeras at ed.ac.uk
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