[BioC] Beadarray data manipulation
Mike Smith
grimbough at gmail.com
Tue Mar 23 22:42:07 CET 2010
Hi Ramzi,
The beadarray package is designed to handle Illumina array data at the
bead-level. Most of its functionality is directed at performing
preprocessing and QA at the bead-level, before the data is summarized by
probe type. These summarized values can then be passed to packages such
a limma for differential expression analysis.
I hope that's of some help,
Mike Smith
Ramzi TEMANNI wrote:
> Hello,
> I have 2 one color Beadarray(HumanRef8 v3) and i'm intrested in comparing
> diffirentially expressed genes at bead level.
> Raw file is a 4 column text file
> Code Grn GrnX GrnY 10008 750 1376.448 8700.565 10008 682 1781.302
> 12418.69 10008 510 1555.273 6437.563 10008 714 910.3025 1081.659 There's
> also an annotation file (bgx)
> I would like to generate a table
> sample 1 sample 2 bead 1 bead 2 …. bead m1 bead 1 bead 2 …. bead
> m2 probeid 1 probeid 2 ….
> probeid
> n
>
> any one had the chance to do this before or have an idea on how to do it ?
>
> Thanks in advance.
> Regards,
> Ramzi
>
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