[BioC] data normalization

[James W. MacDonald]

Hi Barbara,

Barbara Uszczynska wrote:
> Dear R-Users,
> 
> I use home-made spotted arrays to do some research contected with alergies.
> The matrix consist of : 60% of the genes are up-regulated and 40% of genes
> that are down-regulated and spikes. I didn't use any genes with constant
> expression. How I should analyse this experiment?  According to statistics I
> should focuse on external spike  controls and compare all genes with spikes.
> It is two coulour experiment.  So I have to build quite complicated
> statistical model.  I'm not sure if it is a right pathway. What do you
> think?

I think two things:

First, asking the same question over and over will not endear you to the 
listserv community, and will increase the likelihood that your posts 
will simply be deleted by those who might help you.

Second, what you are asking for is statistical help in analyzing your 
experiment rather than help using software. Since many of the people on 
this list are practicing statisticians, what you are asking is for them 
to do what they get paid to do for you for free. I would suggest that a 
more reasonable approach is to find a local statistician to help you 
with your analysis, as you are unlikely to get any (reasonable) help on 
a listserv.

Best,

Jim


> 
> Regards,
> 
> Barbara
> 
> 	[[alternative HTML version deleted]]
> 
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-- 
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826