[BioC] Creating CDF
James W. MacDonald
jmacdon at med.umich.edu
Fri Jul 17 14:46:06 CEST 2009
naresh kumar wrote:
> Dear Dr.MacDonald,
>
> Thank you very much for your reply,
>
> I generated new probeset names by doing custom mapping of probes to
> Genome.The output file is in csv format i need that csv file converted
> to cdf.Is this possible through
> 'makecdfenv' package ? otherwise if possible please provide me any kind
> of information to do it..
No. You need to use the affxparser package:
http://article.gmane.org/gmane.science.biology.informatics.conductor/21824/match=create+cdf
Best,
Jim
>
> I'm displaying few rows of my file to be clear.
> Probe Set Name Probe X Probe Y Probe Interrogation Position Probe
> Sequence Target Strandedness Alignment Chromosome Transcript exon
> NewProbe Indication
> 1007_s_at:718:317; 718 317 3330 CACCCAGCTGGTCCTGTGGATGGGA
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
> 1007_s_at:1105:483; 1105 483 3443 GCCCCACTGGACAACACTGATTCCT
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
> 1007_s_at:584:901; 584 901 3512 TGGACCCCACTGGCTGAGAATCTGG
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
> 1007_s_at:192:205; 192 205 3563 AAATGTTTCCTTGTGCCTGCTCCTG
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
> 1007_s_at:844:979; 844 979 3570 TCCTTGTGCCTGCTCCTGTACTTGT
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
> 1007_s_at:537:971; 537 971 3576 TGCCTGCTCCTGTACTTGTCCTCAG
> Antisense 1 6 uc003nrv.1 exon16 chr6probe447 s
>
>
>
> Thanks and Regards
> Naresh
>
> --- On *Fri, 17/7/09, James W. MacDonald /<jmacdon at med.umich.edu>/* wrote:
>
>
> From: James W. MacDonald <jmacdon at med.umich.edu>
> Subject: Re: [BioC] Creating CDF
> To: "naresh kumar" <naresh4836 at yahoo.co..in>
> Cc: bioconductor at stat.math.ethz.ch
> Date: Friday, 17 July, 2009, 2:26 AM
>
> Hi Naresh,
>
> naresh kumar wrote:
> > Dear mailing list,
> >
> > I got trouble in creating custom cdf in bio conductor.Could any
> one suggest me one tool or source which creates CDF.
>
> Assuming that you want a cdf package for a custom CDF (where you
> actually have the CDF from Affy), you want the makecdfenv package.
>
> Best,
>
> Jim
>
>
> >
> >
> > Thanks for your help in advance
> > Naresh
> >
> >
> >
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> -- James W. MacDonald, M.S.
> Biostatistician
> Douglas Lab
> University of Michigan
> Department of Human Genetics
> 5912 Buhl
> 1241 E. Catherine St.
> Ann Arbor MI 48109-5618
> 734-615-7826
>
>
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--
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
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