[BioC] RMA normalization issue
Fuying Gao
fygao at ucla.edu
Mon Jul 13 19:48:08 CEST 2009
Dear List,
I got error as follows when normolizing a AffyBatch Object (400
samples) with R 2.9.0. It works on an old version of R, R 2.6.1, on
another computer but the computer can not handle so many samples due
to memory issue.
There is no problem to read in the cel files from both versions of R.
Error in rma(dat) :
VECTOR_ELT() can only be applied to a 'list', not a 'character'
#dat is the AffyBatch Object
#session info is as follows (got the above error):
> sessionInfo()
R version 2.9.0 (2009-04-17)
x86_64-redhat-linux-gnu
attached base packages:
[1] splines tools stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] hgu133acdf_2.2.0 annaffy_1.12.1 KEGG.db_2.2.0
[4] GO.db_2.2.0 RSQLite_0.6-8 DBI_0.2-4
[7] AnnotationDbi_1.4.3 gplots_2.6.0 gdata_2.4.2
[10] gtools_2.5.0 MASS_7.2-46 RColorBrewer_1.0-2
[13] affyPLM_1.16.0 simpleaffy_2.16.0 gcrma_2.12.1
[16] matchprobes_1.12.0 genefilter_1.20.0 survival_2.35-4
[19] affy_1.18.1 preprocessCore_1.2.0 affyio_1.8.0
[22] marray_1.18.0 limma_2.14.4 Biobase_2.2.2
loaded via a namespace (and not attached):
[1] annotate_1.18.0
#session info is as follows (no error, an older version R):
> sessionInfo()
R version 2.6.1 (2007-11-26)
i386-pc-mingw32
attached base packages:
[1] splines tools stats graphics grDevices utils
datasets methods base
other attached packages:
[1] hgu133plus2cdf_2.0.0 annaffy_1.10.0 KEGG_2.0.0 GO_2.0.0
[5] gplots_2.3.2 gdata_2.3.1 gtools_2.4.0
MASS_7.2-38
[9] RColorBrewer_1.0-1 affyPLM_1.14.0 simpleaffy_2.14.02
gcrma_2.10.0
[13] matchprobes_1.10.0 genefilter_1.16.0 survival_2.34
affy_1.16.0
[17] preprocessCore_1.0.0 affyio_1.6.0 marray_1.16.0
limma_2.12.0
[21] Biobase_1.16.0
It would be great if someone could help figure out what caused the error.
Thank you!
Fuying Gao
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