[BioC] PCR Validation threshold in dChip normalized data

[Benjamin Otto]

Hi,

Given a dataset for Affymetrix arrays normalized with mas5 or rma we usually
made the experience that signals below 80 (6,3 in log2 format) are hard to
validate with PCR. 

Can somebody tell me, how I can judge on dChip normalized data in a analog
way? Where can I draw a threshold to tell, which signal has good chances to
withstand a verification with with PCR or even on protein level? And which
signals usually indicate a much to low expression level?


Best regards,


Benjamin
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Benjamin Otto
University Hospital Hamburg-Eppendorf
Institute For Clinical Chemistry
Martinistr. 52
D-20246 Hamburg

Tel.: +49 40 42803 1908
Fax.: +49 40 42803 4971
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