[BioC] simpleaffy pairwise.comparison problem
David Ruau
David.Ruau at rwth-aachen.de
Wed Jul 16 15:45:28 CEST 2008
Hi Iain,
I am experiencing the same problem as you on a ExpressionSet generated
from justRMA()
Did you found a solution?
Here is the session:
> da.rma <- justRMA(celfile.path="CELfiles/" , destructive=TRUE,
phenoData=pd)
> da.rma
ExpressionSet (storageMode: lockedEnvironment)
assayData: 54675 features, 214 samples
element names: exprs, se.exprs
phenoData
rowNames:
[...]
> class(pd)
[1] "data.frame"
> pData(da.rma) <- pd
> results <- pairwise.comparison(da.rma, "group", c("A","B"))
Error in validObject(.Object) :
invalid class "PairComp" object: invalid object for slot "pData" in
class "PairComp": got class "factor", should be or extend class
"data.frame"
> sessionInfo()
R version 2.7.1 (2008-06-23)
i386-apple-darwin9.3.0
locale:
en_US.UTF-8/en_US.UTF-8/C/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] splines tools stats graphics grDevices datasets
utils methods base
other attached packages:
[1] simpleaffy_2.16.0 gcrma_2.12.1 matchprobes_1.12.0
genefilter_1.20.0 survival_2.34-1
[6] hgu133plus2cdf_2.2.0 affy_1.18.2 preprocessCore_1.2.0
affyio_1.8.0 Biobase_2.0.1
loaded via a namespace (and not attached):
[1] AnnotationDbi_1.2.2 DBI_0.2-4 RSQLite_0.6-9
annotate_1.18.0 tcltk_2.7.1
On Feb 4, 2008, at 12:50 PM, IAIN GALLAGHER wrote:
> Hi List.
>
> I'm trying to use the simpleaffy pairwise.comparison procedure on
> two groups of arrays. The groups are defined in the phenoData slot
> under the heading 'group' and are indicated by numbers i.e.
> 1,1,2,2,2,3,3,3 etc.
>
> If I try:
>
> results<-pairwise.comparison(test, 'group',c(1,4))
>
> where 'test' is an appropriate subset of the data I get the
> following error:
>
> Error in validObject(.Object) :
> invalid class "PairComp" object: 1: invalid object for slot
> "members" in class "PairComp": got class "numeric", should be or
> extend class "character"
> invalid class "PairComp" object: 2: invalid object for slot "pData"
> in class "PairComp": got class "integer", should be or extend class
> "data.frame"
>
> Is it possible to change the class of the 'group' indicators in my
> phenoData slot or is there another way to get around this problem?
>
> Thanks
>
> Iain
>
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