[BioC] biomaRt difference from biomart.org?
Jorrit Boekel
jorrit.boekel at ki.se
Thu Dec 18 15:08:57 CET 2008
Dear list,
I have tried to fetch 1000bp upstream sequences for a number of rat
genes via biomart. When using the webbased biomart this seems to work
alright, but when automating the process I ran into a problem: for some
genes, "Sequence unavailable" is returned.
Exemplifiyng for a single gene that has this problem:
> sqmrtjb<-useMart("ensembl","rnorvegicus_gene_ensembl")
> getSequence(id="ENSRNOG00000005152",
type="ensembl_gene_id",seqType="coding_gene_flank", upstream=1000,
mart=sqmrtjb)
V1 V2
1 Sequence unavailable ENSRNOG00000005152
On the web, I select ("Attributes->Sequences->Flank(gene), upstream
1000bp, and some associated gene data (Ensembl Gene id, assoc. gene name).
Am I unknowingly querying for different things? Or is the biomaRt
interface connected to a different mart?
thanks,
jorrit
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