[BioC] how to 'bootstrap' expression data?

[Kasper Daniel Hansen]

On Sep 25, 2007, at 3:48 PM, Johnstone, Alice wrote:

> Hi,
> I have a set of Affymetrix array data 3 groups  (two treatment one
> control) of 5 individuals in each.  I have analysed by fitting my  
> model
> with a contrast of treatment-control, and treatment2-control to the
> linear model, using an eBayes correction, then listed the top genes
> using topTable.  I have been asked to 'bootstrap' my results to
> 'weed-out' false positives before QPCR validation.
> Is this a necessary step?  And if so at what stage do I use it and how
> do I go about applying it to my expression data to find the
> differentially expressed genes?
> My background is definitely not in statistics so any discussion or
> pointers would be gratefully received!

Why don't you ask the people who told you to do this: they should be  
in a prime position to know what they mean.

> Thank you
>
> Alice
>
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So do you think this applies to an email list?

Kasper