[BioC] symbol annotation using probes2tableBM
James W. MacDonald
jmacdon at med.umich.edu
Tue Sep 25 22:24:55 CEST 2007
Hi Mayte,
This is a bug that has been fixed in the devel version of affycoretools.
The problem comes from the fact that the 'symbol' descriptor is
different in the human vs mouse (hgnc_symbol vs markersymbol).
Best,
Jim
Mayte Suarez-Farinas wrote:
> Hi,
>
> I am Trying to get mouse4302 affy ID annotation from BioMart using
> probes2tableBM function:
>
> probes2tableBM(fileset,
> probids=ids,filename='test',species="mmusculus",
> ann.source="affy_mouse430_2", links=linksBM()[c(1:3,5)],
> otherann=annBM()[1:2], affyid=TRUE, express=FALSE)
>
> but the column Symbol do not appear in the output.
>
> If I do the following:
> mart=useMart("ensembl", dataset="mmusculus_gene_ensembl")
> Checking attributes and filters ... ok
> > otherann <- annBM(mart)
> > otherann
> [1] "Description" "GO" "GOID" "Chromosome" "ChromLoc"
>
>
> so, symbol do not appear in theannBM output either.
> but if I get the BM annotation directly, the symbol is available:
>
> getBM(c("affy_mouse430_2","entrezgene","markersymbol","refseq_dna"),
> filters="affy_mouse430_2", values="1442021_at", mart=mart)
> affy_mouse430_2 entrezgene markersymbol refseq_dna
> 1 1442021_at 14680 Gnal NM_177137
>
>
> so, I guess the problem could be in AnnB..
>
>
>
>
> [[alternative HTML version deleted]]
>
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