[BioC] print layout for Agilent data?
Jianping Jin
jjin at email.unc.edu
Mon Oct 29 20:19:21 CET 2007
Dear list,
When I was trying the normalizeWithinArrays function of limma I got an
error: Error in switch (method, loess = {: Layout argument not specified).
I was using an Agilent 4 x 44k data set and knew the Agilent printer has
only one block (Row and Col having been taken already). How can I do loess
normalization for each slide? Should I add a Block column into the RG, the
data read-in file in order to do normalizaWithinArrays?
Another question is that is there any easy way I can remove all control
spots from all data files?
Appreciate your help!
JJ
##################################
Jianping Jin Ph.D.
Bioinformatics scientist
Center for Bioinformatics
Room 3133 Bioinformatics building
CB# 7104
University of Chapel Hill
Chapel Hill, NC 27599
Phone: (919)843-6105
FAX: (919)843-3103
E-Mail: jjin at email.unc.edu
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