[BioC] Agilent 4x44 single color expression arrays

Ido M. Tamir tamir at imp.univie.ac.at
Thu Oct 4 12:13:34 CEST 2007


Dear list,

is there anybody with experience in normalising Agilent
4x44 single color expression arrays?

Any suggestions on preferred normalization methods,
or at least reading it into limma in a way that I can
separate the 4 different conditions (time course) that
are on each array?
I have three biological replicates split on 3 arrays and
each array contains 4 time points.

Maybe I should just pipe it through VSN, but with
Affymetrix I was quite happy with GCRMA and would
actually like to apply a probe model based normalization
method.

thank you very much for your suggestions,
ido



More information about the Bioconductor mailing list