[BioC] how to do quantile normalization under dye swape situation
yanju
yanju at liacs.nl
Fri May 11 18:46:29 CEST 2007
Hello All,
my microarray datasets are like follows. And I want to normalize the data.
Targets:
Sample FileName Dye Stage Cy3 Cy5
ZWS57 57.gpr T5C3 high oblong ref high
ZWS58 58.gpr T3C5 high oblong high ref
ZWS61 61.gpr T5C3 high oblong ref high
ZWS62 62.gpr T3C5 high oblong high ref
ref is the commen reference. First I think Within Array Normalization is
not neccesary (am i right?). Then I want to do the Between Array
Normalization using "quantile" method to insure the commen reference
have the same distribution. But with dye swap, I can not use neither
"Rquantile" nor "Gquantile". What should I do? Or other normalization
suggestions?
Regards,
Yanju Zhang
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