[BioC] OT: Problems with Nimblegen scans?

J.delasHeras at ed.ac.uk J.delasHeras at ed.ac.uk
Wed Jun 27 02:51:18 CEST 2007

Hi list,

this is not strictly BioC, but I know there is quite a few people here  
using Nimblegen arrays.
Has anybody else experienced problems with their scans? In particular,  
I received data that was scanned with settings giving rise to a  
significantly larger amount of saturated features than expected/wanted.
We had done an experiment comprising 4 hybs, in triplicate (12 chips).  
The first batch was very good (well, except from a duff hyb... which  
they didn't tell me, and only when I started enquiring -my data looked  
weird- they acknowledged the problem and re-hybed it). But the past  
month we did the other two sets, and their scans are too strong.

Anybody else?


Dr. Jose I. de las Heras                      Email: J.delasHeras at ed.ac.uk
The Wellcome Trust Centre for Cell Biology    Phone: +44 (0)131 6513374
Institute for Cell & Molecular Biology        Fax:   +44 (0)131 6507360
Swann Building, Mayfield Road
University of Edinburgh
Edinburgh EH9 3JR

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