[BioC] Limma and R

[VERONICA COSTANTINI]

Hi, I am trying to run limma under R following the User guide. Everything Ok until I get the message at the end, and I can figure out wich one is the error. Could anyone help me?

Thanks
Veronica


> library (limma)
> dir()
 [1] "Data GPR"                           "ExpDescFile.xls"                   
 [3] "M1.4 VI2.4 (5-10-07) VI"            "M1.8 VI1.8 (5-25-07) VI"           
 [5] "M2.4 VI1.4 (5-10-07) VI"            "M2.8 VI2.8 (5-25-07) VI"           
 [7] "M3.4 VI3.4 (5-10-07) VI"            "M3.8 VI3.8 (5-25-07) VI"           
 [9] "Microarray data -Project M vs V 2"  "Microarray data -Project M vs VI"  
[11] "Microarray data -Project M vs VI 2" "nonM14VI14.gpr"                    
[13] "nonM14VI14.txt"                     "nonM18VI18.gpr"                    
[15] "nonM24VI24.gpr"                     "nonM28VI28.gpr"                    
[17] "nonM34VI34.gpr"                     "nonM38VI38.gpr"                    
[19] "PoSaVsample.txt"                    "PoSaVsampleII.txt"                 
[21] "Test.xls"                           "Verification"                      
> targets <- readTargets("PoSaVsample.txt")
> ls()
[1] "targets"
> targets
  Slide.Number Name      File.Name   Cy3  Cy5
1            1 MV14 nonM14VI14.gpr virus mock
2            2 MV24 nonM24VI24.gpr virus mock
3            3 MV34 nonM34VI34.gpr virus mock
4            4 MV18     nonM18VI18 virus mock
5            5 MV28     nonM28VI28 virus mock
6            6 MV38     nonM38VI38 virus mock
> RG <-read.maimages(targets$File.Name, source="genepix")
Error in RG[[a]][, i] <- obj[, columns[[a]]] : 
        number of items to replace is not a multiple of replacement length
> 

Veronica Costantini
Graduate Research Associate
Food Animal Health Research Program
Dept of Veterinary Preventive Medicine
The Ohio State University
Wooster, Ohio, 44691
Phone: (330) 263-3744
Fax: (330) 263-3677