[BioC] plate image processing and analysis with R

[Wolfgang Huber]

Dear Christian,

such a functionality could certainly be developed on top of EBImage, but
I am not aware of any instances.

If you have a rectangular grid, then maybe also some of the image
analysis software that was developed for quantitating spotted microarray
images could be adapted to your case?

Please also note that .jpg may not always be ideal for quantitative
analyses, you most likely want to avoid compression and want to use grey
scale intensities that are proportional to what the CCD camera measured.

Best wishes
 Wolfgang

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Wolfgang Huber  EBI/EMBL  Cambridge UK  http://www.ebi.ac.uk/huber



> Hi,
> 
> I am looking for a R library that would allow the analysis of images  
> of plates with colonies arranged on a regular grid on it, for  
> instance 32 rows X 48 columns or other densities. These are typically  
> used for large scale screen in yeast genetics and genomics where  
> different colonies are plated using a pin tool and growth rate are  
> estimated based on colony size or intensity from a .jpg image of the  
> plate. Has anyone seen this developed for R/Bioconductor? The  
> functions would serve to locate a grid on the plate and then extract  
> colony intensities or area, in an approach similar to the analysis of  
> microscopy screen available through EBIimage.
> 
> Thanks for letting me know,
> 
> Christian
>