[BioC] Wow, what have I done
Loren Engrav
engrav at u.washington.edu
Sun Dec 9 00:19:01 CET 2007
Ok
But R.app does not show justGCRMA package
But via google I find it in gcrma
So I do
> Gibran81 <- justGCRMA() #81 .cel files
And voila I get
Computing affinities.Done.
Adjusting for optical
effect......................................................................
............Done.
Adjusting for non-specific
binding.....................................................................
............Done.
Normalizing
Calculating Expression
> objects()
[1] "Gibran81" "affinity.spline.coefs"
It did take 25" but then what is 25"?
And
justGCRMA(..., filenames=character(0),
widget=getOption("BioC")$affy$use.widgets,
compress=getOption("BioC")$affy$compress.cel,
celfile.path=getwd(),
sampleNames=NULL,
phenoData=NULL,
description=NULL,
notes="",
normalize=TRUE,
bgversion=2, affinity.info=NULL,
type=c("fullmodel","affinities","mm","constant"),
k=6*fast+0.5*(1-fast), stretch=1.15*fast+1*(1-fast),
correction=1, rho=0.7, optical.correct=TRUE,
verbose=TRUE, fast=TRUE, minimum=1, optimize.by = c("speed","memory"),
cdfname = NULL, read.verbose = FALSE)
Is rather scary, especially k
But assuming "default" is Ok, it is finished, I have gcrma of 81 .cel files,
thank you very much
Also please see new thread in R-Mac on R.app ?bug, thank you
Loren Engrav
Univ Washington
Seattle
From: Sean Davis <sdavis2 at mail.nih.gov>
Date: Sat, 8 Dec 2007 13:26:23 -0500
To: Loren Engrav <engrav at u.washington.edu>
Cc: "bioconductor at stat.math.ethz.ch" <bioconductor at stat.math.ethz.ch>
Subject: Re: [BioC] Wow, what have I done
On Dec 8, 2007 11:36 AM, Loren Engrav <engrav at u.washington.edu> wrote:
>
> And most of our previous stuff is gcrma and not sure mixing is a good idea
> if this is mixing
You might look at justGCRMA(), the GCRMA.
Sean
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