[BioC] summarizing probe intensites before or after normalization- 1. how to do with RMA 2. Opinions?

Ben Bolstad bmb at bmbolstad.com
Mon Sep 11 15:51:46 CEST 2006


> 1. Help to effect Bolstad normalization of the RMA preprocessed and 
> summarized data. Whilst I succeed in generating unnormalized RMA 
> preprocessed data with-
> 
> library(affy)
> data <- ReadAffy()
> datarma <- rma(data, normalize=FALSE)
> 
> As a result of my limited R experience, I failed in finding a method to 
> effect Bolstad (quantile) normalization on this output.

library(affyPLM)
datarma.postqnorm <- normalize(datarma)



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