[BioC] probe level annotation
Shi, Tao
shidaxia at yahoo.com
Mon Oct 2 22:00:33 CEST 2006
Hi list,
I found the XY coordinates for the probes in 'hgu133aprobe' are not exactly the same as those shown on NetAffx.com. Please see below.
Also, is the order of the probes from pm(myAffyData, '1007_s_at'), for examples, the same as in 'hgu133aprobe'?
thanks,
...Tao
> R.Version()
$platform
[1] "i386-pc-mingw32"
$arch
[1] "i386"
$os
[1] "mingw32"
$system
[1] "i386, mingw32"
$status
[1] ""
$major
[1] "2"
$minor
[1] "3.0"
$year
[1] "2006"
$month
[1] "04"
$day
[1] "24"
$`svn rev`
[1] "37909"
$language
[1] "R"
$version.string
[1] "Version 2.3.0 (2006-04-24)"
> library(hgu133aprobe)
> data(hgu133aprobe)
> as.data.frame(hgu133aprobe[1:16,])
sequence x y Probe.Set.Name Probe.Interrogation.Position Target.Strandedness
1 CACCCAGCTGGTCCTGTGGATGGGA 467 181 1007_s_at 3330 Antisense
2 GCCCCACTGGACAACACTGATTCCT 531 299 1007_s_at 3443 Antisense
3 TGGACCCCACTGGCTGAGAATCTGG 86 557 1007_s_at 3512 Antisense
4 AAATGTTTCCTTGTGCCTGCTCCTG 365 115 1007_s_at 3563 Antisense
5 TCCTTGTGCCTGCTCCTGTACTTGT 207 605 1007_s_at 3570 Antisense
6 TGCCTGCTCCTGTACTTGTCCTCAG 593 599 1007_s_at 3576 Antisense
7 TCCTGTACTTGTCCTCAGCTTGGGC 425 607 1007_s_at 3583 Antisense
8 ACTTGTCCTCAGCTTGGGCTTCTTC 552 101 1007_s_at 3589 Antisense
9 TCCTCCATCACCTGAAACACTGGAC 680 607 1007_s_at 3615 Antisense
10 AAGCCTATACGTTTCTGTGGAGTAA 532 139 1007_s_at 3713 Antisense
11 TTGGACATCTCTAGTGTAGCTGCCA 143 709 1007_s_at 3786 Antisense
12 TCTCTAGTGTAGCTGCCACATTGAT 285 623 1007_s_at 3793 Antisense
13 GTGTAGCTGCCACATTGATTTTTCT 383 479 1007_s_at 3799 Antisense
14 GCCACATTGATTTTTCTATAATCAC 129 279 1007_s_at 3807 Antisense
15 TACACTAATATATGGACCTAGCTTG 62 651 1007_s_at 3871 Antisense
16 ATATATGGACCTAGCTTGAGGCAAT 308 15 1007_s_at 3878 Antisense
> package.version('hgu133aprobe')
[1] "1.12.0"
And from NetAffx.com
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position Target Strandedness
CACCCAGCTGGTCCTGTGGATGGGA 197 207 3330 Antisense
GCCCCACTGGACAACACTGATTCCT 592 319 3443 Antisense
TGGACCCCACTGGCTGAGAATCTGG 85 591 3512 Antisense
AAATGTTTCCTTGTGCCTGCTCCTG 559 139 3563 Antisense
TCCTTGTGCCTGCTCCTGTACTTGT 269 633 3570 Antisense
TGCCTGCTCCTGTACTTGTCCTCAG 378 629 3576 Antisense
TCCTGTACTTGTCCTCAGCTTGGGC 536 643 3583 Antisense
ACTTGTCCTCAGCTTGGGCTTCTTC 479 125 3589 Antisense
TCCTCCATCACCTGAAACACTGGAC 575 643 3615 Antisense
AAGCCTATACGTTTCTGTGGAGTAA 497 161 3713 Antisense
TTGGACATCTCTAGTGTAGCTGCCA 635 739 3786 Antisense
TCTCTAGTGTAGCTGCCACATTGAT 74 659 3793 Antisense
GTGTAGCTGCCACATTGATTTTTCT 660 503 3799 Antisense
GCCACATTGATTTTTCTATAATCAC 80 313 3807 Antisense
TACACTAATATATGGACCTAGCTTG 739 685 3871 Antisense
ATATATGGACCTAGCTTGAGGCAAT 242 31 3878 Antisense
More information about the Bioconductor
mailing list