[BioC] RLE question for QC

[Wolfgang Raffelsberger]

Hi,

with good experiments I see an IQR of apr -0.2 to +0.2 . 
But I rather look if all the arrays of this study have homogeneous RLE 
IQRs and medians, since typical RLE IQR also reflect (in part) the 
nature of the samples tested.  Basically, if your samples (and so the 
corresponding fluorescence signal intensities) are very much alike you 
tend to get smaller RLE IQRs.  So there may be an explanation based on 
the experimental details to consider !

Keep in mind that - although the RLE is indeed very useful - it's not 
the sole QC criteria.

Cheers,
Wolfgang

D.Enrique ESCOBAR ESPINOZA a écrit :
> HI,
>
> In RLE boxplots we expect to see values centered on zero showing
> small inter-quartile range.
>
> Do anyone know what threshold is widely acceptable?
> (for median or IQR values)
>
> Thanks.
>
>
>  
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Wolfgang Raffelsberger, PhD
Laboratoire de BioInformatique et Génomique Intégratives
IGBMC
1 rue Laurent Fries,  67404 Illkirch  Strasbourg,  France
Tel (+33) 388 65 3314         Fax (+33) 388 65 3276
wolfgang.raffelsberger at igbmc.u-strasbg.fr