[BioC] background correction
yanju
yanju at liacs.nl
Tue Nov 28 16:06:13 CET 2006
Dear all,
As read from the instruction of backgroundCorrect in limma, the method
"half", "minimum", "edwards" and so on are all designed to produce
positive corrected intensities. My code is as follows:
rgc<-backgroundCorrect(rg, method="half")
plotDensities(rgc)
After I plot the graph of Density-Intensity after this backgroud
correction, I found there is still some part of curve with the negative
intensity. Why is it like that?? I try minimun also, the result is
similar with negative intensity. I dont know how to explain it.
Expecting your reply.
Regards,
Yanju
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