[BioC] rflowcyt and prada questions

[Nolwenn LeMeur]

Hi Andras,

Maybe you can send us (Florian Hahne and myself) one of your time serie 
FCS file?
We will try to solve your problems.

Best,
Nolwenn


**************************************
Nolwenn Le Meur, PhD
Fred Hutchinson Cancer Research Center
Computational Biology
1100 Fairview Ave. N., M2-B876
P.O. Box 19024
Seattle, WA 98109-1024

On Thu, 25 May 2006, dr. Treszl András wrote:

> Hi everyone,
> 
> I am using the rflowcyt package to analyze data generated on a FACSAria 
> machine with the DiVa software. I collect data for 10 minutes after an 
> initial stimulation of the cells than I export the FCS 3.0 files to 
> rflowcyt. After reading in the data I noticed that the data on 
> channel "Time" do not correspond to the actual duration of the measurement. 
> Namely, when I collected data for 10 minutes (=600 sec), my time-scale in 
> rflowcyt spans to 250 "units", instead of 600. (I am calculating a lot of 
> statistics, thus the proper time-resolution would be very important.) 
> However, if I try to analyze the same data with another software (e. g. 
> FlowJo), the scale is correct, thus the data needed are within the file, 
> simply I am not able to extract it. What should I do? How can I get the 
> proper time-resolution?
> 
> My second question: I have never been able to read in my FCS3.0 file from 
> the DiVa software via the prada package. Does prada recognize the DiVa-
> derived FCS3.0 files? If not, do you have an extension for it?
> 
> Your help is greatly appreciated,
> 
> best
> 
> Andras
> 
> Andras Treszl MD PhD
> First Department of Pediatrics, Semmelweis University, Budapest, Hungary
> Bókay u. 53
> H-1083 Budapest, Hungary
> treszl at gyer1.sote.hu
> Tel.: 36/1/334-3186
> Fax.: 36/1/313-8212
> 
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