[BioC] Trouble calculating fold-changes - please help
Srinivas Iyyer
srini_iyyer_bio at yahoo.com
Sat May 13 01:54:30 CEST 2006
Hello:
I am analyzing farmer_breast data. I classified
normals as normal and any other transformed tissue
into cancer.
in my class file CL0 = normal and CL1 = Cancer
I wrote a function to compute fold changes.
I would like some help from group members to comment
on this function to calculate fold changes.
1. is there a better way to calculate.
2. how do i get log-fold changes.
fc <- function(x,CL0,CL1){
x0 <- x[,CL0]
x1 <- x[,CL1]
res <- (apply(x0,1,mean) - apply(x1,1,mean))
}
foldcs
<-fc(mydata_mat,CL0=(farm.cl==0),CL1=(fram.cl==1))
logfoldcs <- log(foldcs,2) OR loggoldcs <-
log2(foldcs)
While doing this I get error:
Warning message:
NaNs produced in: log(x, base)
Could any one help me please.
Thanks
sri
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