[BioC] Minor correction to: degraded RNA and background correction

[Jenny Drnevich]

I should be more precise about the prokaryotic labeling method... 
Fragmented cDNA is labeled at the 3' end, then hybridized to antisense 
arrays. Affy's manual only says the labeling is done with GeneChip DNA 
Labeling Reagent, but the original reference used 70 µM biotin-N6-ddATP 
(http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=11713332] 


At 01:43 PM 2/28/2006, Jenny Drnevich wrote:
>  Is it possible that prokaryotic RNA and
>its N-terminus labeling method are different enough from eukaryotic RNA and
>the biotin-labeled nucleotide labeling method so that these functions
>(particularly gc-based bg correction) should not be used?
>
>Thanks in advance,
>Jenny
>
>
>Jenny Drnevich, Ph.D.
>
>Functional Genomics Bioinformatics Specialist
>W.M. Keck Center for Comparative and Functional Genomics
>Roy J. Carver Biotechnology Center
>University of Illinois, Urbana-Champaign
>
>330 ERML
>1201 W. Gregory Dr.
>Urbana, IL 61801
>USA
>
>ph: 217-244-7355
>fax: 217-265-5066
>e-mail: drnevich at uiuc.edu
>
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