[BioC] nested design in limma?
georg.otto at tuebingen.mpg.de
Fri Feb 24 10:44:51 CET 2006
Gordon Smyth <smyth at wehi.edu.au> writes:
> Just as an aside, I am continually amazed at how common technical
> dye-swaps are. As far as I can see, they just complicate the analysis
> to no advantage, yet they have captured the imagination of many
> biologists. My guess is that this an attempt to balance the dyes,
> although this can be better achieved without introducing technical replication.
This is an interesting point, since I am going to design and analyse
two-colour experiments soon and I am interested in what people think
about it. Do you recommend dye-swapping with biological replicates? Or
no dye-swapping at all?
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