[BioC] justRMA using incorrect default cdf

Kimpel, Mark William mkimpel at iupui.edu
Mon Feb 6 05:57:43 CET 2006


I am using justRMA to analyze Affy rat2302 chips. I do have installed on
my system the alternative MBNI cdf package but do not want to use it in
this case. For some reason, just.rma and justRMA are using the MBNI cdf
as the default cdf file and using the parameter  "cdfname = 'rat2302'"
does not work to over-ride. Below is my code and sessionInfo. Note that
I am using the devel versions or R and BioC with all latest package
updates installed.

Thanks, Mark

> affy.object.preprocessed$eSet <-just.rma(filenames=list.celfiles(),
phenoData = read.pData.ordered.func("pdata.3.txt"))

Background correcting
Normalizing
Calculating Expression

> affy.object.preprocessed$eSet
Expression Set (exprSet) with 
        10255 genes
        30 samples
                 phenoData object with 2 variables and 30 cases
         varLabels
                Condition: read from file
                Treatment: read from file
> annotation(affy.object.preprocessed$eSet)
[1] "rn230rnentrezg6"

> sessionInfo()
Version 2.3.0 Under development (unstable) (2006-01-01 r36947) 
i386-pc-mingw32 

attached base packages:
[1] "splines"   "tools"     "methods"   "stats"     "graphics"
"grDevices" "utils"     "datasets"  "base"     

other attached packages:
rn230rnentrezg6cdf      affycoretools            GOstats
multtest         genefilter           survival             xtable
RBGL 
           "6.0.0"            "1.3.1"            "1.5.4"
"1.8.0"            "1.9.2"             "2.20"            "1.3-0"
"1.7.7" 
          annotate                 GO              graph
Ruuid            cluster              limma               affy
Biobase 
           "1.9.2"            "1.6.5"            "1.9.9"
"1.9.0"           "1.10.2"            "2.4.9"            "1.9.7"
"1.9.6" 
>

Mark W. Kimpel MD



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