[BioC] HGU133 spike-in

[Talloen, Willem [PRDBE]]

hi all,

I observed in the Affy latin square experiment more noise between the
triplicates of probe sets spiked in at lower concentrations. This is
apparent with all summarization techniques used like MAS5 and (GC-)RMA. Did
somebody else also detect this tendency?

I am looking for an explanation for it. Are these concentrations made in a
dilution serie? If so, this increased variation with decreasing
concentration might result from the fact that lower concentrations
experienced more dilution steps. Or not?

Cheers,
Willem