[BioC] urgent..
James W. MacDonald
jmacdon at med.umich.edu
Mon Apr 17 14:58:07 CEST 2006
Actually, if the data are .csv files then they most likely have already
been processed using either MAS5 or GCOS. In that case you won't be able
to do RMA or GCRMA. For that you need the .CEL files.
However, as Naomi noted the first few sections of the affy vignette
should help get you going if/when you get the celfiles.
Best,
Jim
Naomi Altman wrote:
> You are in luck. All you need to do is read the first few sections
> of the affy manual, and you will see that this is easily done in 4 or
> 5 lines of code.
>
> --Naomi Altman
>
> At 03:50 PM 4/16/2006, kst wrote:
>
>>hi,
>> I need to do summarizations of the probe intensity values for
>>each probeset of Affymetrix data in excel(.csv) format, by RMA,
>>GCRMA and MAS5. Can you please suggest me how to do it in R? or is
>>there any code available?
>> Thank-you for your help.
>> kavita
>>
>>
>>---------------------------------
>>
>>
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>
> Naomi S. Altman 814-865-3791 (voice)
> Associate Professor
> Dept. of Statistics 814-863-7114 (fax)
> Penn State University 814-865-1348 (Statistics)
> University Park, PA 16802-2111
>
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--
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623
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