[BioC] Dealing with technical replication with a common reference design
smyth at wehi.edu.au
Sat Oct 1 08:55:47 CEST 2005
At 08:43 PM 12/09/2005, michael watson \(IAH-C\) wrote:
>When I called duplicateCorrelation, I got three warning messages:
>Too much damping - convergence tolerance not achievable in: glmgam.fit(
>My corfit$consensus was then positive, even though it is a dye-swap
>experiment. Presumably this means I don't have to treat the technical
>replicates in any special way...?
That would be my interpretation, if everything else is correct. Technical
variation often dominates biological. If the correlation is much larger
than zero however, something is wrong.
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