[BioC] limma reading wrong column for GPR files

Guoneng Zhong guoneng.zhong at yale.edu
Thu May 26 21:25:16 CEST 2005


Hi,

 I have files generated by Genepix 3.0 and when I use maimages 
(source="genepix") and checked the RG value (RG being the object created 
by read.maimages), the red channel foreground reading and the 
greenchannel foreground reading are swapped (incorrectly), but the 
background channel are not swapped.  What gives?  Does the latest 
version of limma know how to read Genepix v. 3 (as opposed to version 
5/6, which have different column orderings).

Thanks,
G



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