[BioC] affycomp, *.CEL and *.CSV files
Mohammad Esad-Djou
shahrgol at web.de
Thu May 19 12:23:00 CEST 2005
Hello all,
I use affycomp library, in order to test different Methods.
I can produce *.csv file from *.CEL files:
library(affy)
library(affycomp)
library(affydata)
library(tools)
data.raw <- ReadAffy(filenames="./R/ME_cel/Expt1_R1.CEL",
...
"./R/ME_cel/Expt14_R1.CEL")
# read process finished
eset <- mas5(data.raw)
x <- exprs(eset)
write.table(data.frame(x,check.names=FALSE),file="H:/Profile/Eigene Dateien/Dip_bak/Pr_sprach/R/excel/ME_133_1.csv",sep=",",col.names=NA,quote=FALSE)
Now I concentrated on 2 questions:
1. How can I get "Statndard data.frame" without Pre processing?
I can accomplish this data.frame with affycomp further tests.
(description in: http://affycomp.biostat.jhsph.edu/#whatisthis)
2. as I above file (ME_133_1.csv) for read.spikein to use wants, gets I error message:
spikein133.ngenes <- 0
f <- "H:/Profile/Eigene Dateien/Dip_bak/Pr_sprach/R/excel/ME_133_1.csv"
s <- read.spikein(f,"hgu133a")
Error in "[.data.frame"(s, , rownames(pData(pd))) :
undefined columns selected
How can I correct my errors?
Thanks,
Mohammad Esad-Djou
More information about the Bioconductor
mailing list