[BioC] affycomp, *.CEL and *.CSV files

Mohammad Esad-Djou shahrgol at web.de
Thu May 19 12:23:00 CEST 2005


Hello all,

I use affycomp library, in order to test different Methods.  
I can produce *.csv file from *.CEL files:

library(affy)
library(affycomp)
library(affydata)
library(tools)

data.raw <- ReadAffy(filenames="./R/ME_cel/Expt1_R1.CEL",
...					
"./R/ME_cel/Expt14_R1.CEL")

# read process finished
eset <- mas5(data.raw)
x <-  exprs(eset)
write.table(data.frame(x,check.names=FALSE),file="H:/Profile/Eigene Dateien/Dip_bak/Pr_sprach/R/excel/ME_133_1.csv",sep=",",col.names=NA,quote=FALSE) 

Now I concentrated on 2 questions:  

1.  How can I get "Statndard data.frame" without Pre processing? 

I can accomplish this data.frame with affycomp further tests.  
(description in:  http://affycomp.biostat.jhsph.edu/#whatisthis) 

2. as I above file (ME_133_1.csv) for read.spikein to use wants, gets I error message:

spikein133.ngenes <- 0
f <- "H:/Profile/Eigene Dateien/Dip_bak/Pr_sprach/R/excel/ME_133_1.csv"
s <- read.spikein(f,"hgu133a")
Error in "[.data.frame"(s, , rownames(pData(pd))) : 
        undefined columns selected

How can I correct my errors?

Thanks,
Mohammad Esad-Djou



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