[BioC] Limma final gene expression report
Ankit Pal
pal_ankit2000 at yahoo.com
Tue May 10 07:15:17 CEST 2005
Dear All,
While looking at the Limma user guide, I came across
the following example
> targets <- readTargets("SwirlSample.txt")
> RG <- read.maimages(targets$FileName, source="spot")
> RG$genes <- readGAL()
> RG$printer <- getLayout(RG$genes)
> MA <- normalizeWithinArrays(RG)
> MA <- normalizeBetweenArrays(MA)
> fit <- lmFit(MA, design=c(-1,1,-1,1))
> fit <- eBayes(fit)
> options(digits=3)
> topTable(fit, n=30, adjust="fdr")
ID Name M A t P.Value B
control BMP2 -2.21 12.1 -21.1 0.000357 7.96
control BMP2 -2.30 13.1 -20.3 0.000357 7.78
control Dlx3 -2.18 13.3 -20.0 0.000357 7.71
control Dlx3 -2.18 13.5 -19.6 0.000357 7.62
fb94h06 20-L12 1.27 12.0 14.1 0.002067 5.78
fb40h07 7-D14 1.35 13.8 13.5 0.002067 5.54
I have omitted a few rows and columns.
Here we see that after all the data transformations,
we get an output where the ranking for the probes in
an array is done on the basis of the B value.
Notice that there are reapeating names for genes,
therefore for a set of replicates, within and across
arrays, each spot is reported separately as an
individual entity.
In the case of BMP2 from the above example, which
result do I consider?
Is there a way in which I can get a single result for
a set of replicates.
I am new to this package, so please do let me know if
there is a problem in my understanding the concept.
Thank you,
-Ankit
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