[BioC] [how to] cel files -> directly to expression level

[Kasper Daniel Hansen]

read.affybatch reads the file into an AffyBatch object. This object is 
very similar to an exprSet, and there is no normalization or anything 
going on. Afterwards you can use exprs() to acces the raw expression 
intensities.

Kasper

On Fri, May 06, 2005 at 08:34:27AM +0200, s_r wrote:
> Hi. 
> 
> I know R language a little so I've got a question to You. I'm asking You for help because i can't find it anywhere. 
> 
> I want to know how to get an expression level of genes from the .CEL file.  
> I've found some information to do it with using a functions like rma or mas to get an object exprs from object of class affybatch. But I need raw expression level before apply any normalization algorithm (expression directly from cel files).  Than I want to save it (expression level) with function write.exprs. 
> 
> 
> Thank You very much, and sorry for my English...
> Sebastian
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> 
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-- 
Kasper Daniel Hansen, Research Assistant
Department of Biostatistics, University of Copenhagen