[BioC] Problems with justGCRMA: Error in bg.adjust.fullmodel

Jordi Altirriba Gutiérrez altirriba at hotmail.com
Thu Aug 25 12:10:23 CEST 2005


Dear users,
I’m trying to obtain the GCRMA expression values of 26 Affymetrix 
microarrays (HGU133plus2). Due to a limitation of RAM (2Gb), I can’t use 
GCRMA directly:

Data<-ReadAffy()
esetGCRMA<-GCRMA(Data)

So I’m trying to do it with justGCRMA, but I’m obtaining this error:

>esetGCRMA<-justGCRMA(filenames=list.celfiles(),type="fullmodel",optimize.by="memory",fast=TRUE)
Computing affinities..Done.
Adjusting for optical effect...........................Done.
Adjusting for non-specific binding.Error in bg.adjust.fullmodel(pms[, i], 
mm, pm.affinities, mm.affinities,  :
        unused argument(s) (Q ...)

I’ve also tried with 
just.gcrma(filenames=list.celfiles),type="fullmodel",optimize.by="memory",fast=TRUE)

But I’m obtaining the same error.

The data of my computer and R:
PC: Intel centrino M 2GHz
OS: Windows XP pro

R version 2.1.0, 2005-06-02, i386-pc-mingw32

attached base packages:
[1] "tools"     "methods"   "stats"     "graphics"  "grDevices" "utils"  
"datasets"  "base"

other attached packages:
        vsn       plier       gcrma matchprobes        affy  reposTools
    "1.6.3"     "1.0.0"     "1.1.3"    "1.0.22"     "1.6.7"    "1.5.19"
    Biobase
   "1.5.12"

Any help will be very much appreciated.

Thanks for your time

Jordi Altirriba
PhD student
Hospital Clinic (Barcelona, Spain)



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