[BioC] limma Function

[Jianping Jin]

Dear BioConductor list:

I started to learn limma function for giving weight to all Genepix flags 
less than -50. Also I tried to filter out spots that, for example, show the 
foreground/background ratio less than 1.2. I am wondering whether or not:
1. I can set two different weight values in my data set, e.g. 0.5 for 
$Flags == -50 and 0.1 for $Flags == -100 with a function?
2. I can perform flag weighting and forground/background weighting 
described above at the same time when reading data in? Or are there any 
other ways to do that?

I will appreciate it if you could help me out!

JP-


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x Jianping Jin Ph.D.            x
x Bioinformatics scientist      x
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